FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12971053
Sequences flagged as poor quality0
Sequence length53-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTAG11950.022.485092120
TCTAGGT13950.018.791634120
TCTAGGG10600.017.846891116-117
GTCTAGG11100.016.968071114-115
CGTCTAG14750.016.439493120
CTAGGTG9900.012.683206114-115
GATCTAG14750.012.217967118-119
TCGAGTA40450.011.341181120
AAAAAAA633700.010.776128120
ATCTAGG13450.010.719109118-119
CTAGGGA8350.010.2947108-109
GTCTAGA16250.010.045099114-115
CGTATCA266700.09.01824120
TGGTCGC416950.08.880619120
GAGAGTC42000.08.582071120
GTGGTCG449700.08.379607120
GCGTCGG96450.08.2217045120
CGTAGTG44800.08.1919775120
CCGTATC320500.08.097405120
GGTCGCC408800.08.047696120
Produced by FastQC (version 0.11.5)