FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13029838
Sequences flagged as poor quality0
Sequence length50-126
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC211700.0176.65266120
GAGCACA377250.098.38617120
AGAGCAC516700.072.22538118-119
AAGAGCA768750.046.632576118-119
CTTCCGA156700.044.956021
CCGATCT155650.044.5618674
GAAGAGC846050.044.087887116-117
CGATCTA44550.041.1933635
TCCGATC170150.041.104063
GGAAGAG1030350.034.822433116-117
TTCCGAT228000.031.0339432
CGGAAGA1230000.030.524023114-115
GATCTAG14000.029.932386
CACGTCT56650.029.13368120
ACACGTC55200.027.905704120
TCGGAAG1289550.027.86095114-115
CACACGT48900.026.569878118-119
CGATCTC72300.025.8489845
ATCGGAA1502250.025.198685112-113
GATCGGA1518000.023.804089112-113
Produced by FastQC (version 0.11.5)