FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12192841
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC93750.060.234188120
GAGCACA142300.041.221573120
AGAGCAC191650.032.140602120
AAGAGCA331850.019.372154120
GAAGAGC369300.018.355938120
GGAAGAG465350.014.916599120
TCGGAAG537150.013.132279120
GATCGGA625600.012.844967120
CGGAAGA558550.012.567939118-119
ATCGGAA628550.012.008646120
AGATCGG685050.011.739384120
TAGATCG205550.010.952459120
CTAGATC47400.010.422582120
TTAGATC87150.09.185067118-119
AAGATCG275100.08.683591120
GAGATCG254200.08.192101120
CAGATCG238000.07.554412118-119
TAAGATC93700.07.3414135120
CCTAGAT20100.07.000439116-117
TGAGATC94450.06.8860655118-119
Produced by FastQC (version 0.11.5)