FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11405460
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC130300.069.95769120
GAGCACA191650.043.27151120
AGAGCAC255350.034.114624118-119
AAGAGCA393850.022.661306120
GAAGAGC448800.020.628864120
GGAAGAG550000.016.61069120
TCGGAAG646800.014.214289118-119
CGGAAGA660400.014.163235120
GATCGGA744450.012.349919116-117
ATCGGAA752550.012.32087116-117
AGATCGG818600.011.3306875114-115
TAGATCG176550.010.472709118-119
CACGTCT43850.010.231173120
CTAGATC43600.09.744316118-119
TTAGATC76250.09.271528118-119
CACACGT39700.09.074928118-119
AAGATCG375400.09.008516114-115
GAGATCG275250.08.594218114-115
CCTAGAT14750.08.295419118-119
TCTAGAT21350.08.277936108-109
Produced by FastQC (version 0.11.5)