FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15897663
Sequences flagged as poor quality0
Sequence length61-126
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG72100.011.008391120
GTGGTCG300400.010.068233120
TCTCGGT459450.09.933188120
ATCTCGG506550.09.67432120
CTCGGTG438150.09.633841120
AGATCTC577300.09.6031685120
GATCTCG550000.09.544138120
TAGATCT622500.09.176339120
CGGTGGT379650.08.932662120
TGGTCGC269000.08.829375120
GGTCGCC272750.08.752073120
GGTGGTC333050.08.647912120
TCGGTGG396700.08.639684120
GAGTGTA911300.08.617216120
AGTGTAG857750.08.482226120
GTAGATC798500.08.388715120
GTGTAGA815550.08.360797120
GCCGTAT227600.07.9256377120
AGAGTGT982200.07.866624120
TGTAGAT865300.07.8314543120
Produced by FastQC (version 0.11.5)