FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15897663
Sequences flagged as poor quality0
Sequence length50-126
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC166550.079.06533120
GAGCACA268100.047.139526120
AGAGCAC361400.037.254135120
AAGAGCA583350.022.700195120
GAAGAGC605750.022.054234118-119
GGAAGAG742400.018.578283120
CGGAAGA852350.016.173878120
TCGGAAG893150.015.430149118-119
ATCGGAA1038250.013.355497120
GATCGGA1078550.012.87403116-117
AGATCGG1193800.011.6311655118-119
TAGATCG332150.010.640167116-117
AAGATCG498650.010.040948120
ACACGTC47900.09.94843120
CACACGT42000.09.26852120
GAGATCG345800.09.193301118-119
CTAGATC79750.09.071976112-113
ACGTCTG56750.08.751803120
TTAGATC163100.08.687937116-117
CAGATCG314500.08.450963120
Produced by FastQC (version 0.11.5)