FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18527130
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC206600.071.762024120
GAGCACA313550.046.370094120
AGAGCAC432450.036.225296120
AAGAGCA678100.022.589369118-119
GAAGAGC749050.020.672974118-119
GGAAGAG918700.018.01786120
TCGGAAG1071800.015.297983118-119
CGGAAGA1075500.015.162501120
ATCGGAA1273850.012.865866120
GATCGGA1296650.012.660977116-117
AGATCGG1402500.011.576446118-119
CTAGATC72600.011.00055120
TAGATCG303150.010.707003118-119
AAGATCG604100.09.034093116-117
ACACGTC56550.08.811613120
GAGATCG509350.08.751285118-119
TTAGATC133150.08.561507116-117
CACACGT49450.08.420322120
CAGATCG481500.08.179837120
CACGTCT59100.07.969421120
Produced by FastQC (version 0.11.5)