FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13897975
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC201050.087.20826120
GAGCACA318000.050.54516120
AGAGCAC426050.039.562855118-119
AAGAGCA638500.026.911747120
GAAGAGC732200.022.9245116-117
GGAAGAG859800.019.396362118-119
CGGAAGA1006950.016.79989114-115
TCGGAAG1029100.016.12611116-117
ATCGGAA1189400.014.329171112-113
GATCGGA1214900.013.795555114-115
AGATCGG1343150.012.652868110-111
ACACGTC61000.012.08817120
CTAGATC75200.011.588617118-119
TAGATCG297700.011.583968120
CACACGT55700.010.831411120
CACGTCT56150.010.744605120
GAGATCG507700.010.424373110-111
TTAGATC106600.010.410805120
CCTAGAT23700.09.900553114-115
CAGATCG437350.09.810487114-115
Produced by FastQC (version 0.11.5)