FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15401658
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC191000.079.65822120
GAGCACA290750.050.059395120
AGAGCAC400600.039.448166120
AAGAGCA618500.025.921556120
GAAGAGC682000.023.286879118-119
GGAAGAG841200.018.364546118-119
CGGAAGA990800.016.17727116-117
TCGGAAG1001850.015.773753120
ATCGGAA1181100.013.570603114-115
GATCGGA1187500.013.114626118-119
AGATCGG1299950.012.310398112-113
CTAGATC69850.011.81013120
TAGATCG319050.011.507032116-117
CACGTCT52850.011.265653120
TTAGATC114700.09.80538116-117
CACACGT54050.09.688364120
AAGATCG490700.09.481692114-115
GAGATCG448450.09.288775114-115
CAGATCG416150.09.019801110-111
ACACGTC55100.08.852798120
Produced by FastQC (version 0.11.5)