FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13523581
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC97300.047.617058120
GAGCACA146400.034.074974120
AGAGCAC177250.027.12406120
GAAGAGC329900.017.522013120
AAGAGCA340900.015.072552120
GGAAGAG427000.014.180057120
TCGGAAG446050.013.364753120
CGGAAGA483350.012.39138118-119
TAGATCG132150.012.221351120
AGATCGG586150.012.127818120
GATCGGA559300.011.372133120
ATCGGAA574050.010.721462120
AAGATCG291500.08.332071118-119
TAACCCC26000.07.7374669
CTAGATC35050.07.650099120
TTAGATC75600.07.5884423120
GAGATCG237650.07.3600216118-119
CAGATCG242600.06.2845197118-119
CTACCCC18000.06.081131538-39
AACCCCC18850.05.963969710-11
Produced by FastQC (version 0.11.5)