FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13629310
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC191800.086.731125120
GAGCACA309200.053.15862120
AGAGCAC425550.038.952583118-119
AAGAGCA622800.026.863438120
GAAGAGC720700.023.397818120
GGAAGAG859500.020.22628120
CGGAAGA1025400.016.74863118-119
TCGGAAG1049900.016.186634118-119
ATCGGAA1215500.014.186494116-117
GATCGGA1247100.013.727042116-117
AGATCGG1369900.012.525221114-115
TAGATCG269600.011.991678120
CACACGT43550.011.472761120
CTAGATC65100.011.378153112-113
TTAGATC102800.010.864198120
GCTAGAT26050.010.55735112-113
AAGATCG585900.010.471133114-115
CACGTCT49800.010.3279915120
GAGATCG454900.09.511698110-111
CAGATCG433300.09.394382120
Produced by FastQC (version 0.11.5)