FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14544856
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC227800.092.34962120
GAGCACA354650.056.645714120
AGAGCAC477600.042.88349118-119
AAGAGCA676000.029.888302118-119
GAAGAGC788950.025.880903120
GGAAGAG976550.020.796417118-119
CGGAAGA1165500.017.481789114-115
TCGGAAG1177500.017.247028116-117
CACGTCT78400.015.880675120
ATCGGAA1361100.015.128591114-115
CACACGT79300.015.092682120
GATCGGA1417900.014.649793114-115
ACACGTC81200.013.948143120
AGATCGG1525000.013.581196112-113
ACGTCTG87350.013.0580635120
TAGATCG410300.012.770717110-111
CGTCTGA96400.012.748754120
GAGATCG631250.011.622596112-113
CTAGATC72750.011.572835112-113
GCACACG93700.011.297124118-119
Produced by FastQC (version 0.11.5)