FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14397353
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC137550.064.764824120
GAGCACA210500.041.710392120
AGAGCAC280500.032.491203120
AAGAGCA450750.020.261868120
GAAGAGC483150.019.115652120
GGAAGAG597050.015.963426120
TCGGAAG688850.013.929221120
CGGAAGA706550.013.698366120
ATCGGAA829150.012.779806120
GATCGGA832450.012.145511118-119
AGATCGG917500.011.318341120
TAGATCG255600.010.453088116-117
CTAGATC65700.08.987374120
GAGATCG360750.08.303137118-119
TTAGATC115850.08.102349116-117
AAGATCG358900.08.042494116-117
CCTAGAT29900.07.3923264112-113
CAGATCG304300.07.316777118-119
ATAGATC140250.06.258743116-117
ACACGTC41250.06.057698118-119
Produced by FastQC (version 0.11.5)