FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12915054
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC141950.062.214413120
GAGCACA221700.040.096313120
AGAGCAC296650.031.943232120
AAGAGCA454500.020.466208118-119
GAAGAGC494350.019.585796120
GGAAGAG602750.017.250553120
TCGGAAG715850.014.394466118-119
CGGAAGA711850.014.389353120
ATCGGAA861400.012.684422120
GATCGGA855450.012.373266120
AGATCGG949250.011.7482120
TAGATCG217350.011.210836120
CTAGATC57600.010.184128118-119
TTAGATC95250.09.8808365120
AAGATCG427300.09.836046120
GAGATCG344750.09.180854120
CACGTCT45300.08.538054120
CACACGT39050.08.418894120
CCTAGAT22550.08.004292110-111
ACACGTC46050.07.979048120
Produced by FastQC (version 0.11.5)