FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13706661
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC169450.068.37523120
GAGCACA262750.045.91319120
AGAGCAC356300.034.900604120
AAGAGCA533600.024.11201120
GAAGAGC609750.020.665659118-119
GGAAGAG763600.018.143456120
TCGGAAG904700.014.522032118-119
CGGAAGA916950.014.208684118-119
ATCGGAA1062750.012.817898120
GATCGGA1083450.012.726033120
CACACGT50500.011.950814120
AGATCGG1201250.011.4615118-119
TAGATCG259150.010.3901615118-119
CACGTCT53300.010.327575120
CTAGATC60950.010.119446120
GTCTAGA7656.565456E-59.536274120
TTAGATC100300.09.521581120
AAGATCG513650.09.4125595120
ACACGTC56400.09.171978120
GAGATCG433900.08.987422120
Produced by FastQC (version 0.11.5)