FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14116684
Sequences flagged as poor quality0
Sequence length61-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTGT222400.09.993795120
GTAGGGA414000.09.895724120
AGTGTAG185250.09.890202120
GAGTGTA195950.09.871295120
TAGGGAA391950.09.53286120
GTGTAGA199800.09.500689120
TAGATCT133850.09.469513120
CGTGTAG533500.09.052847120
TGTAGGG460300.09.0439120
AAGAGTG262000.09.010623120
GTGTAGG504350.08.778069120
AGATCTC154000.08.5035305120
GTAGATC211200.08.475901120
TCGTGTA577050.08.421678120
GTCGTGT610800.08.054682120
CGTCGTG688800.07.7356120
AGGGAAA457200.07.673087120
TGTAGAT238300.07.6380434120
AGCGTCG774500.07.414809120
AAAGAGT329750.07.3596992120
Produced by FastQC (version 0.11.5)