FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14116684
Sequences flagged as poor quality0
Sequence length50-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC131500.045.61976120
GAGCACA172550.035.779697120
AGAGCAC229850.030.03765120
AAGAGCA368750.018.604628120
GAAGAGC406600.017.164444120
TCGGAAG555000.014.73444120
GGAAGAG501550.014.437736120
CGGAAGA571200.012.983254120
ATCGGAA662650.012.265411120
GATCGGA668500.012.176753120
AGATCGG759250.011.091301120
TAGATCG200150.010.884917120
CTAGATC58600.09.826614118-119
TTAGATC85550.08.768661116-117
GAGATCG331500.07.3802114118-119
CCTAGAT23350.07.332124114-115
AAGATCG322350.07.116657116-117
ATAGATC103750.06.618505120
CAGATCG313950.06.1837974120
GCTAGAT26700.06.178754116-117
Produced by FastQC (version 0.11.5)