FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15085277
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC121900.064.56212120
GAGCACA188800.042.899094120
AGAGCAC258600.033.41291120
AAGAGCA431200.020.378082120
GAAGAGC477750.018.378769118-119
GGAAGAG590500.016.120714120
TCGGAAG690400.013.806513120
CGGAAGA722650.012.926027120
GATCGGA794750.012.306191120
ATCGGAA814900.011.751857120
AGATCGG876600.011.484007120
TAGATCG257000.011.124391120
CTAGATC58550.010.331413120
GAGATCG354100.08.865107120
TTAGATC95200.08.594469118-119
AAGATCG301650.07.9999776118-119
CCTAGAT24200.07.63037120
CAGATCG300950.07.5956173120
ATAGATC140850.07.4591627120
TGAGATC143800.07.0847425120
Produced by FastQC (version 0.11.5)