FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15832246
Sequences flagged as poor quality0
Sequence length50-126
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC228100.0132.75974120
GAGCACA396350.072.90624120
AGAGCAC545850.054.406162118-119
AAGAGCA834450.035.037037118-119
GAAGAGC916800.032.390938116-117
GGAAGAG1104400.026.441032116-117
CGGAAGA1268750.023.383017114-115
CCGATCT75850.021.9341624
TCGGAAG1326600.021.918169114-115
CGATCTA27900.019.5122995
ATCGGAA1542700.019.379225112-113
ACACGTC56450.019.09796120
GATCGGA1587150.018.41299112-113
TCCGATC94150.018.3733123
CACGTCT60650.018.251556120
AGATCGG1707050.017.555426110-111
CACACGT51700.016.995825118-119
TAGATCG525150.015.797711110-111
CAGATCG554900.014.570055110-111
CTTCCGA117350.014.4123751
Produced by FastQC (version 0.11.5)