FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12846908
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC139800.072.708595120
GAGCACA217900.047.111988120
AGAGCAC298950.034.384262120
AAGAGCA475100.022.132046120
GAAGAGC517600.020.444927120
GGAAGAG619450.017.117725118-119
TCGGAAG748600.014.909957120
CGGAAGA756200.014.635401120
GATCGGA877300.012.6650715118-119
ATCGGAA891850.012.545314120
CTAGATC51850.011.822145120
AGATCGG958300.011.619111118-119
TAGATCG198950.010.930026116-117
AAGATCG451150.09.610137116-117
TTAGATC87900.09.444946114-115
GAGATCG299300.08.98347116-117
TAAGATC184100.08.305691120
CAGATCG275000.08.279187120
CCTAGAT17100.08.255524114-115
CAAGATC237550.07.8546786120
Produced by FastQC (version 0.11.5)