FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14395139
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC148200.061.290314120
GAGCACA226850.042.737103120
AGAGCAC299900.033.357933120
AAGAGCA475650.021.008091118-119
GAAGAGC523800.019.01108120
GGAAGAG626800.016.642576120
TCGGAAG737400.013.950185120
CGGAAGA739450.013.828924118-119
GATCGGA876750.012.595671120
ATCGGAA869900.012.03705120
AGATCGG976050.011.116362118-119
TAGATCG237200.010.528569114-115
CACACGT49100.010.448632120
CACGTCT56800.09.84276120
CTAGATC54400.09.483276118-119
GAGATCG425050.09.214969118-119
ACACGTC63000.08.874108120
AGAGATC182800.08.484411116-117
TTAGATC84700.08.45753114-115
CAGATCG343550.08.021774120
Produced by FastQC (version 0.11.5)