FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14289783
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC122450.057.421844120
GAGCACA184050.038.374546120
AGAGCAC241850.031.68044120
AAGAGCA397550.021.049421120
GAAGAGC423050.018.88626120
GGAAGAG527700.015.487299118-119
TCGGAAG618450.013.377972120
CGGAAGA647050.013.059543120
GATCGGA734950.012.038181120
ATCGGAA736200.012.017742120
AGATCGG815450.011.553518120
TAGATCG213250.010.94142120
CTAGATC54800.09.493516116-117
TTAGATC100250.08.806461118-119
AAGATCG340450.08.33528120
GAGATCG292500.08.22482118-119
CACACGT50650.07.9682236120
CACGTCT49700.07.359234120
TAAGATC117950.07.00381120
CAGATCG297800.06.9773173118-119
Produced by FastQC (version 0.11.5)