FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15387713
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC157550.062.615276120
GAGCACA237600.041.92844120
AGAGCAC312050.034.18757120
AAGAGCA513100.021.309301120
GAAGAGC561450.019.263683118-119
GGAAGAG694750.015.914895120
TCGGAAG803700.014.53117120
CGGAAGA812100.014.285153120
GATCGGA931750.012.99993120
ATCGGAA941750.012.79311120
AGATCGG1028150.011.72745118-119
TAGATCG255600.010.79562120
CTAGATC66450.010.527557120
AAGATCG425350.09.504716116-117
GCTAGAT31350.09.091047120
GAGATCG318500.08.948331120
TTAGATC111150.08.758731118-119
CAGATCG326850.08.549502116-117
TAAGATC183250.08.1652120
ATAGATC111650.07.0102296116-117
Produced by FastQC (version 0.11.5)