FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11642393
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC122100.062.01204120
GAGCACA190150.042.772823120
AGAGCAC254700.032.4142120
AAGAGCA393650.022.64525120
GAAGAGC450300.018.921942120
GGAAGAG538750.016.32473118-119
CGGAAGA640550.014.843742120
TCGGAAG647650.014.2325115120
ATCGGAA765950.012.64109120
GATCGGA776350.012.247346118-119
AGATCGG874150.011.17979120
TAGATCG204550.010.508445120
CACGTCT47750.010.273867120
CTAGATC54150.09.954529114-115
TTAGATC79500.09.580951120
CCTAGAT19950.09.059596120
CACACGT40650.08.81311118-119
GAGATCG369650.08.574019120
CGCTAGA8502.401066E-108.353283106-107
AAGATCG323800.08.033968116-117
Produced by FastQC (version 0.11.5)