FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15173998
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC162200.072.69239120
GAGCACA242900.046.148518120
AGAGCAC326450.036.71144120
AAGAGCA515350.022.932732120
GAAGAGC568650.021.05685118-119
GGAAGAG698850.017.426048120
TCGGAAG825550.014.696852118-119
CGGAAGA831450.014.663579120
GATCGGA953350.012.817653120
ATCGGAA969200.012.658014120
AGATCGG1048600.011.689325118-119
TAGATCG282500.011.45315114-115
CTAGATC67850.010.300107120
GAGATCG357350.09.681584120
TTAGATC106500.09.282999110-111
ACTAGAT30700.09.240931120
AAGATCG341250.08.883623116-117
CACGTCT53450.08.673559120
CAGATCG379800.08.655605110-111
CCTAGAT22900.08.604181112-113
Produced by FastQC (version 0.11.5)