FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16110312
Sequences flagged as poor quality0
Sequence length50-126
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC283700.0106.66161120
GAGCACA467000.062.05315120
AGAGCAC631650.047.080868118-119
AAGAGCA946300.031.225489118-119
GAAGAGC1022600.028.645084116-117
GGAAGAG1231650.023.614534116-117
CACGTCT86450.023.19892120
CACACGT82550.022.796564120
ACACGTC83250.021.968124120
CGGAAGA1431700.020.574081114-115
TCGGAAG1487400.019.539299114-115
ACGTCTG105350.017.695171120
ATCGGAA1769350.017.027367112-113
GATCGGA1810550.016.376394112-113
GCACACG108300.015.785538118-119
AGATCGG1948900.015.429188110-111
CGTCTGA123400.013.889719120
TAGATCG451900.013.66601112-113
GAGATCG736450.013.226417110-111
CTAGATC90100.013.188772112-113
Produced by FastQC (version 0.11.5)