FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12908312
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC157650.083.52944120
GAGCACA246300.051.132587120
AGAGCAC330150.038.88772118-119
AAGAGCA500550.026.013622120
GAAGAGC572550.022.626556120
GGAAGAG702050.018.665054118-119
CGGAAGA808000.015.975846114-115
TCGGAAG816000.015.923173116-117
CACGTCT53900.014.073901120
ATCGGAA943700.013.850204112-113
GATCGGA961500.013.6741905114-115
AGATCGG1051550.012.460358110-111
CACACGT53100.012.205461120
ACACGTC57150.011.598245120
TAGATCG195150.010.751117118-119
AAGATCG472200.010.562161112-113
GAGATCG323350.09.710045116-117
CAGATCG336000.09.574402112-113
ACGTCTG65450.09.45226120
TAAGATC212150.09.33846120
Produced by FastQC (version 0.11.5)