FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15927583
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC187750.077.76338120
GAGCACA304750.049.96753120
AGAGCAC426050.038.24842120
AAGAGCA662300.024.226778118-119
GAAGAGC765200.021.152102118-119
GGAAGAG923650.017.830683120
TCGGAAG1103400.015.96056120
CGGAAGA1082650.015.397023120
ATCGGAA1295250.013.80783120
GATCGGA1351650.013.206978120
AGATCGG1489300.011.903383118-119
TAGATCG382350.011.296645120
CTAGATC95350.010.432102120
CACACGT44100.010.293821118-119
ACACGTC54650.09.894666120
TTAGATC132400.09.6809845118-119
GAGATCG562250.09.26129116-117
CAGATCG565800.09.232678118-119
AAGATCG428600.09.143073120
CACGTCT54200.08.498771120
Produced by FastQC (version 0.11.5)