FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15455186
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC242200.0111.72444120
GAGCACA401350.065.670105120
AGAGCAC540850.049.48163118-119
AAGAGCA793250.032.880745118-119
GAAGAGC907850.029.565828116-117
CCGATCT123500.028.8801254
TCCGATC127250.028.766653
CTTCCGA148100.024.8579981
CGATCTA30300.024.0932485
GGAAGAG1093800.024.019833116-117
CGGAAGA1292600.020.918262114-115
TCGGAAG1340350.019.629713114-115
TTCCGAT210500.017.838252
ATCGGAA1547950.017.552967112-113
GATCGGA1604850.016.435352112-113
CACGTCT65700.016.387997120
GATCTAG9250.016.3292836
ACACGTC67750.015.629445120
AGATCGG1725500.015.609984110-111
CACACGT63400.015.2983120
Produced by FastQC (version 0.11.5)