FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11359144
Sequences flagged as poor quality0
Sequence length50-126
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC205950.0152.9502120
GAGCACA353050.088.85099120
AGAGCAC484350.064.80999118-119
AAGAGCA701900.043.569366118-119
GAAGAGC778150.040.11607116-117
GGAAGAG925050.032.681393116-117
CGGAAGA1060100.029.406303114-115
CACGTCT53300.028.113358120
CACACGT44950.027.739237120
TCGGAAG1149600.026.236818114-115
ACACGTC53550.024.305628120
ATCGGAA1317450.023.950258112-113
CGTCTGA64500.022.55336120
GATCGGA1389450.021.977007112-113
ACGTCTG59000.021.318918120
GCACACG52700.021.273188118-119
AGATCGG1504300.021.085682110-111
TAGATCG353350.019.132969110-111
GAGATCG588550.018.28058110-111
CAGATCG444050.017.516071110-111
Produced by FastQC (version 0.11.5)