FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19243786
Sequences flagged as poor quality0
Sequence length54-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA2865000.012.100177120
GCGTCGG136250.011.330834120
TCTCGGG42850.09.953431120
CGTATCA1027900.09.531656120
CTAGGGT9001.3481622E-69.3341141
CTTGGGG45750.08.787621
CCGTATC1148450.08.740367120
GTATCAT1039850.08.682676120
GCCGTAT1233100.08.578796120
CGCCGTA1340950.08.511504120
GGTCGCC1645750.08.172511120
CTTCCGA97050.08.1614251
TGGTCGC1730700.08.080282120
TCGCCGT1491950.08.060743120
TATCATT1171800.07.9459076120
GTCGCCG1608600.07.894457120
GTGGTCG1855300.07.832258120
GGTGGTC1986900.07.7760715120
CGGTGGT2142200.07.711484120
ATCATTA1018850.07.6706533120
Produced by FastQC (version 0.11.5)