FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19243786
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC299250.0105.32852120
GAGCACA508900.060.85761120
AGAGCAC679900.046.060074118-119
AAGAGCA1030000.030.10147118-119
GAAGAGC1126050.027.699446116-117
GGAAGAG1351100.022.941372118-119
CGGAAGA1524250.020.399502114-115
CACGTCT82100.019.756634120
TCGGAAG1610650.019.140514116-117
CACACGT80150.018.445456120
ATCGGAA1858900.016.890165112-113
ACGTCTG89250.016.754055120
ACACGTC85750.016.015137118-119
GATCGGA1959000.015.857301114-115
AGATCGG2165250.014.603838110-111
CGTCTGA109950.013.446143120
TAGATCG533900.013.388457110-111
GAGATCG878650.012.393769112-113
CAGATCG738050.011.666858112-113
CTAGATC94750.011.558535110-111
Produced by FastQC (version 0.11.5)