FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12894092
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC88100.051.437256120
GAGCACA128850.032.705933120
AGAGCAC160150.027.635353120
AAGAGCA286650.016.156353120
GAAGAGC291250.016.136278120
GGAAGAG400300.013.077742120
GATCGGA464900.012.586084120
TCGGAAG412600.012.401209118-119
AGATCGG501050.011.491643118-119
TAGATCG123200.011.317738120
ATCGGAA508850.011.229883120
CGGAAGA459900.011.166391120
CTAGATC33750.010.144047120
AAGATCG267650.09.163287120
TTAGATC65450.08.8449545120
GAGATCG163750.08.210976120
CAGATCG163100.07.1559744118-119
TAAGATC133850.06.278237120
TCTAGAT30650.06.0927343118-119
CAAGATC163750.05.397945118-119
Produced by FastQC (version 0.11.5)