FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11341410
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC155450.086.04441120
GAGCACA250600.050.394615120
AGAGCAC333800.039.810093120
AAGAGCA502450.027.21234120
GAAGAGC581650.023.64409120
GGAAGAG701000.019.619026118-119
CGGAAGA832650.016.573666118-119
TCGGAAG854400.016.03794116-117
ATCGGAA1000600.013.864875116-117
GATCGGA1016500.013.605867114-115
AGATCGG1114450.012.442565114-115
TAGATCG233000.011.8243065116-117
CTAGATC57050.010.928482120
CACACGT41250.010.544946120
AAGATCG431250.09.860705114-115
TTAGATC85450.09.673015114-115
CCTAGAT17000.09.596083108-109
CAGATCG369700.09.580411112-113
GAGATCG394350.09.559573120
ACACGTC45750.09.507737120
Produced by FastQC (version 0.11.5)