FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13103853
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC138800.080.33652120
GAGCACA224150.048.569378120
AGAGCAC307500.035.954414120
AAGAGCA462100.024.437996120
GAAGAGC563800.021.313911120
GGAAGAG672300.017.476238118-119
CGGAAGA798850.015.288231120
TCGGAAG802400.014.693172118-119
ATCGGAA931950.013.075743120
GATCGGA958800.012.850711120
AGATCGG1056650.011.621157118-119
TTAGATC100750.010.74534120
TAGATCG271950.010.644141112-113
CTAGATC65650.09.724021112-113
AAGATCG379750.09.443291120
TAAGATC137950.08.470822116-117
GAGATCG391000.08.427474120
CAGATCG380050.08.296414120
TGAGATC131150.08.059957118-119
CTCTAGA11003.731856E-57.9964266120
Produced by FastQC (version 0.11.5)