FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12278180
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC131700.067.81989120
GAGCACA204250.046.613052120
AGAGCAC260200.035.772038120
AAGAGCA398800.023.808662118-119
GAAGAGC445600.020.894514118-119
GGAAGAG544250.017.492046116-117
TCGGAAG624300.015.124333114-115
CGGAAGA636900.014.752701116-117
GATCGGA743450.012.848201112-113
ATCGGAA742350.012.666712114-115
AGATCGG807150.011.609797110-111
TAGATCG200900.011.361588116-117
CTAGATC47700.09.882489116-117
CCTAGAT16650.09.366423114-115
TTAGATC65450.08.772805116-117
AAGATCG296800.08.550008110-111
GAGATCG313750.08.415693110-111
CAGATCG321200.08.032916110-111
ATAGATC106550.07.5177917114-115
CACGTCT42400.07.362138120
Produced by FastQC (version 0.11.5)