FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14347271
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC137100.055.6868120
GAGCACA201550.039.471054120
AGAGCAC268800.029.736328120
AAGAGCA421350.019.21683118-119
GAAGAGC469800.018.272192120
GGAAGAG584750.015.486856120
TCGGAAG653950.013.53091118-119
CGGAAGA685700.013.051133118-119
GATCGGA791400.012.595176120
ATCGGAA793750.012.209277120
AGATCGG899550.011.171783120
CTAGATC60800.010.447088118-119
TAGATCG221000.010.315569118-119
TTAGATC93450.08.412048120
AAGATCG405850.08.336576120
CCTAGAT22700.08.311375118-119
GAGATCG373200.08.257061120
CACACGT42100.07.9171934118-119
CACGTCT49100.07.300698120
ACACGTC47600.07.134406120
Produced by FastQC (version 0.11.5)