FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11628381
Sequences flagged as poor quality0
Sequence length58-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAT129600.014.120081
GCGTCGG85000.012.003107120
CAATACC100500.011.7609521
CCTTCAC92050.011.6673311
CATTACC88850.011.1421451
CCTTGAT112050.011.1376551
CAACACC101300.010.5427241
CCTGCAC124500.010.3612331
TCTCGGT386650.010.2444725120
AGATCTC499850.010.133663120
TAGATCT527400.010.093626120
CCTTGAC85400.010.0466311
CCTCCAT108200.010.036761
CTCGGTG367400.09.997145120
GGTGGTC282350.09.9901905120
ATCTCGG435200.09.984202120
GGTCGCC231200.09.916062120
GTGGTCG253100.09.911693120
TCGGTGG336950.09.867519120
CCTTACC53550.09.859741
Produced by FastQC (version 0.11.5)