FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14611786
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC192250.081.29577120
GAGCACA310650.050.643864120
AGAGCAC413800.038.233807120
AAGAGCA608600.026.445019120
GAAGAGC689500.023.234375118-119
GGAAGAG821850.019.41185118-119
CGGAAGA954250.016.745972116-117
TCGGAAG975900.016.446482120
ATCGGAA1141300.014.367187120
GATCGGA1182100.013.75218118-119
AGATCGG1312400.012.32285112-113
CACGTCT56500.012.288475120
CACACGT51550.012.2502118-119
TAGATCG216250.012.125171116-117
ACACGTC61400.011.067207120
CTAGATC54500.010.435418116-117
CAGATCG533000.09.949834120
AAGATCG453600.09.7212120
GAGATCG502700.09.579906114-115
ACGTCTG74300.09.443944120
Produced by FastQC (version 0.11.5)