FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16028603
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC225850.078.302956120
GAGCACA380150.048.8852120
AGAGCAC503450.037.233578120
AAGAGCA745950.025.939074120
GAAGAGC859550.022.085415118-119
GGAAGAG1012500.018.95083120
CGGAAGA1198350.016.691635120
TCGGAAG1238750.015.808497118-119
CACGTCT70950.015.34341120
ATCGGAA1458450.013.710033120
GATCGGA1521100.013.063007118-119
CACACGT70850.012.886827120
AGATCGG1696350.011.948775120
ACACGTC78450.011.548865120
TAGATCG417850.011.1042385116-117
CTAGATC104300.010.012745116-117
ACGTCTG81100.09.352881120
CAGATCG725250.09.190639114-115
GAGATCG745200.09.15618116-117
TTAGATC114700.08.982663116-117
Produced by FastQC (version 0.11.5)