FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12685803
Sequences flagged as poor quality0
Sequence length50-126
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC208150.0171.49684120
GAGCACA349600.098.36768120
AGAGCAC474650.074.00678118-119
AAGAGCA697650.048.96206118-119
GAAGAGC768500.045.59967116-117
GGAAGAG917350.037.1796116-117
CGGAAGA1061900.033.1466114-115
TCGGAAG1127450.030.274107114-115
CACACGT49450.028.21586118-119
CACGTCT55300.028.096481120
ACACGTC56100.027.926617120
ATCGGAA1311200.027.084923112-113
GATCGGA1334700.025.79015112-113
AGATCGG1421350.024.75429110-111
CCGATCT57250.023.2876034
TAGATCG342400.023.109816110-111
CGTCTGA76650.021.79081120
ACGTCTG66250.021.353254118-119
TCCGATC67100.020.785033
GAGATCG448000.020.084787110-111
Produced by FastQC (version 0.11.5)