FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15986133
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC237500.0106.32366120
GAGCACA398300.059.399506120
AGAGCAC537450.045.473663118-119
AAGAGCA798650.030.912388120
GAAGAGC922500.027.196186120
GGAAGAG1128050.022.24856118-119
CGGAAGA1333850.018.815825118-119
TCGGAAG1409600.018.077087116-117
ATCGGAA1616100.015.747036116-117
GATCGGA1673900.015.376553114-115
CACGTCT89050.014.678464120
AGATCGG1822700.014.056263114-115
CTAGATC107750.013.496272112-113
CACACGT82550.013.459111120
TAGATCG475700.013.215527114-115
ACACGTC88600.013.185946118-119
GCACACG90750.012.963146120
GAGATCG589000.012.109048112-113
ACGTCTG109300.011.8095120
CAGATCG598900.011.629235112-113
Produced by FastQC (version 0.11.5)