FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19592419
Sequences flagged as poor quality0
Sequence length50-126
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC347750.0127.52058120
GAGCACA605800.073.30741120
AGAGCAC844400.052.740086118-119
AAGAGCA1246950.035.879997118-119
GAAGAGC1409200.031.478193116-117
GGAAGAG1746850.025.446373116-117
ACACGTC84600.022.818602120
CGGAAGA2042600.021.78447114-115
TCGGAAG2100200.021.090717114-115
CACGTCT87050.020.486752120
CACACGT84150.019.827257118-119
ATCGGAA2456400.018.35234112-113
GATCGGA2531300.017.67675112-113
ACGTCTG101000.017.111046120
AGATCGG2736400.016.472773110-111
GCACACG100050.016.170956120
TAGATCG622500.015.284143110-111
CAGATCG912850.014.173891110-111
GAGATCG948000.014.02168112-113
CTAGATC131000.013.859128110-111
Produced by FastQC (version 0.11.5)