FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30406339
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT19300.014.5263581
CGTATCG39400.014.3507823
CCCGTAT50150.013.7391251
GACGCTA43050.013.3497624
GTATCGA45150.012.8340394
ACGCTAC49350.012.0305145
CGCGTAT6553.645073E-810.8820821
CGCTACT62800.010.2858756
TCGACCG20000.09.9742757
GTTCGAT44700.09.8863841
CGCGATT9952.401066E-109.5514411
CCGGGAT91550.09.5503991
CGGTAAT27550.09.4864371
CCGGTAT42000.09.3905451
CGGAAAT73600.09.2970831
CTCGGAT76100.09.1789861
CGGGGAT87600.09.1131221
CGCGTAA5801.2168627E-49.0121151
CGGGGTT100850.08.858171
CCGTATC74550.08.7954152
Produced by FastQC (version 0.11.5)