FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28192103
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT16800.015.2791191
CGCGGGT53750.012.9118411
CGACCTA18500.012.5839274
CGCGTAT7055.820766E-1112.1366041
CCGGTAT48150.010.8595091
CGCGAAT12000.010.6953821
CGCGGAT31600.010.2290581
CGGTATT45450.09.5143412
TATCGCG12400.09.38438120-21
CGCCAAT50200.09.3744381
CGTATCG25450.09.3340193
CGGAAAT65000.09.2144841
CCGGCAT63050.09.1225051
CGGGGAT83000.09.0488381
CGGCAAT39950.09.042961
CCCGTAT39050.08.886191
CCGGGAT82650.08.8571021
GTATCGA28100.08.2847924
CGGGAAT64500.08.2541451
CGGGGTT87550.08.0899141
Produced by FastQC (version 0.11.5)