FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30868305
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG40650.017.7652233
CGACCGT19800.017.5246261
GTATCGA44750.016.2437654
CCCGTAT51150.015.5189341
GACGCTA43900.013.0951124
TCGACCG20500.012.9749717
CGCGTAT6552.237357E-1012.3366911
ACGCTAC51400.011.1820815
CCGGTAT41550.011.0966461
CCGTATC78050.010.4090432
ATCGAAG95550.010.2899186
CGCTACT61900.09.66834456
CGCGAAT11403.6379788E-129.5898991
TCGAAGG103450.09.3663837
CCGACAT60450.09.357111
GTTCGAT45950.09.2065211
CCGCAAT38900.09.0421811
CGCCAAT51050.09.0316491
CGCGTTT20650.08.9770911
TATCGAA89400.08.9263135
Produced by FastQC (version 0.11.5)