FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28042811
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT52850.012.5864521
CGACCGT17050.012.5403151
CGTATCG27850.012.4491313
CCCGTAT37950.011.7689131
CGCGTAT7351.2369128E-1011.6360461
CGACCTA16600.011.4444274
GTATCGA30550.010.7270434
CGCCAAT52000.010.3251271
CCGGTAT46500.010.3202141
CCGGGAT81000.09.1508171
CGCGTGT29850.08.9138221
CGGACAT54850.08.7491321
CGCGAGT25550.08.7403171
CGGGAAT65000.08.6255921
CGCGAAT10052.7570422E-88.5099451
CGGGTAT29700.08.4789041
CGGGGAT83300.08.3847991
CTCGGGT74450.08.29657651
CCGGCAT66750.08.257091
CCGCAAT35250.08.2222571
Produced by FastQC (version 0.11.5)