FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31304417
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT389900.12455111366552521Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT145950.027.1740027
CGTATCA81750.026.32031248-49
ATCGGAA169300.024.0620751
AGCGTCG173000.023.3096039
CGCCGTA86400.022.8420544-45
GCCGTAT97600.022.11876546-47
AAGAGCG181050.022.115826
GAGCGTC187150.021.6740468
TCGGAAG199800.020.9768352
GCGTCGT112750.019.46155510-11
TGGTCGC109750.018.35020340-41
TCGTGTA108900.018.16505812-13
GTCGTGT123200.017.57939312-13
CCGTATC114900.017.30024146-47
CGTGTAG127650.017.19047414-15
GGTCGCC132300.016.92783442-43
TCGCCGT135550.016.22404944-45
GTAGATC137850.016.16077830-31
GTGGTCG144200.015.69557440-41
GAGTGTA148400.014.97866626-27
Produced by FastQC (version 0.11.5)