FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30278598
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG37350.016.4058533
CCCGTAT46250.015.2091151
GTATCGA40450.014.9113444
CGACCGT20950.014.3898041
GACGCTA42500.012.8511074
CCGTATC54050.012.4927522
ACGCTAC48300.011.60285955
CAATCGG62650.011.5226964
CGGTAAT28650.010.6894091
CCGTCAT66000.010.6579031
CGCGGAT30650.010.6163881
AATCGGG70200.010.5540145
TCAATCG66350.09.9511583
CCGCAAT35200.09.5159851
CTTGGAT221700.09.2595521
CCGGGAT83800.09.1363831
CGACGAT23600.09.1242861
CCGGTAT36600.09.0212421
CGAACGA19400.08.93608258-59
CTCGGAT75150.08.9145091
Produced by FastQC (version 0.11.5)