FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24950986
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT19450.025.7573721
GTATCGA32400.018.7649774
CCGTCAT68050.018.5801661
ACCGTTG26300.017.5189363
GACCGTT27500.016.4124432
GACGCTA53950.016.0237064
CCCGTAT46000.015.2472441
ACGCTAC57250.015.181795
TACTCTA81150.014.9244749
CGGTAAT23000.014.5211851
CGTATCG44750.014.1172593
CGTCATC90650.013.259742
CGCTACT66350.013.242756
CGCGGTT20750.012.4167381
CCGTATC58500.011.7759092
CCGGGAT58750.011.6134171
GCGCTAA55250.011.60563442-43
CGAACGA23350.010.8828758-59
AACCCGT45450.010.8713062
GTTCGAT50400.010.6974361
Produced by FastQC (version 0.11.5)